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文献和实验Comparison of Competitive PCR and Positive Control-Based PCR
The exponential amplification of small amounts of nucleic acids makes polymerase chain reaction (PCR) not only powerful but also challenging as a quantitative method. Variations in nucleic acid preparation, thermal cyclic performance
Chromosomal DNA Extraction from Gram-positive Bacteria
extract lysed cells,and ethanol precipitate the DNA with 0.1 vol.3 M sodium acetate,pH 4.8 and 2 vol.95% ethanol. 5.Spool out DNA with a glass rod,wash once with 80% ethanol before drying. Some bacterial species may require a longer incubation
Positive Selection Scanning of Parasite DNA Sequences
to the parasite. Random genetic mutations that confer selective advantage can act to alter amino acids so as to confer gain of function that has positive impact on the survival of the parasite. Directional selection of advantageous mutations results
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