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文献和实验Maintenance of Chicken Embryonic Stem Cells In Vitro
as a fusion protein linked to glutathione S-transferase (GST) and purified to greater than 90% purity in two chro-matography stages, the first an affinity step using the GST tail, which was cleaved before further purification by gel chromatography. Chicken ES
Isolation and Culture of Primary Chicken Osteoclasts
through the use of osteoclasts directly isolated from the bones of chickens fed a low calcium diet to enhance osteoclastogenesis and bone resorption. Particular advantages of this system include the abundance and highly resorptive nature of isolated chicken
Expression of IgA Molecules in Mammalian Cells
antibodies is the lack of well established production and purification technologies. Here, we describe protocols to produce and purify recombinant chimeric IgA antibodies irrespective of their antigen specificity. Resulting antibodies were monomeric, fully
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