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文献和实验Comparison of Competitive PCR and Positive Control-Based PCR
The exponential amplification of small amounts of nucleic acids makes polymerase chain reaction (PCR) not only powerful but also challenging as a quantitative method. Variations in nucleic acid preparation, thermal cyclic performance
Polymerase 0.5 μl 2. Prepare a control reaction with no template DNA and an additional 10 μl of sterile water. 3. If the thermocycler does not have a heated lid, add
Quality Control of Nucleotides and Primers for PCR
The polymerase chain reaction (PCR) has become an essential tool in molecular biology for the rapid amplification and analysis of DNA (1 ). The technique requires the use of oligonucleotide primers that bind to the DNA at a specific region
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