赛默飞世尔Thermo Fisher X2 TRAP. VO

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

Monoclonal Antibody Production Protocol

the PEG. Do not disturb the cells. Spin at 1000 rpm for 5 min. Aspirate medium, resuspend cells by tapping. Slowly add 5 ml of 37°C IMDM-m. Bring to 20 ml and add to the flask in the incubator. (If using feeder cells, add them at this point; 106 cells