6041.5275赛默飞nanoViper 20µm x 6

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

The ribonuclease protection assay (RPA)

by gentle flicking and quick spin in a microfuge. Incubate at 37°C for 30 minutes. 3. Add the following reagents (in order) to each 1.5 ml Eppendorf tube: 26 µl 20 mM EDTA 25 µl Tris-saturated phenol 25 µl chloroform:isoamyl alcohol (50

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide