Sartorius赛多利斯16306Glass-vacuum

SDS-PAGE Western Blotting Protocols

glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening

Preparation of Embryonic Extract

. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml

Preparation of Embryonic Extract

. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter.      15. Cameo 0.22 um filters (Fisher Scientific).      16. 50 ml syringe for applying pressure.      17. 3x 25 ml sterile flasks.      18. 15 ml conical disposable centrifuge tubes