Sartorius赛多利斯16315Glass vacuum

Protocol for vacuum-infiltration transformation of Arabidopsis thaliana

). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day

Green lab protocol for vacuum infiltration transformation of Arabidopsis

, inoculate a 500 ml culture of YEP medium containing 50 mg/l rifampicin, 25 mg/l gentamycin and the appropriate antibiotic for your construct with a 1 ml overnight starter culture. Be sure to water your plants well the day before infiltration

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

at 3000 rpm for 25-30 minutes in a Beckman GS-R centrifuge. Each pellet then is washed with 30 ml of 70% ethanol and dried in vacuum.  10、Each pellet is dissolved in 210 ul of 10/0.1 TE by pipetting up and down and incubating at 37 degrees C followed