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16315Glass vacuum filter holder, 25 mm/30 ml,
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day
Green lab protocol for vacuum infiltration transformation of Arabidopsis
, inoculate a 500 ml culture of YEP medium containing 50 mg/l rifampicin, 25 mg/l gentamycin and the appropriate antibiotic for your construct with a 1 ml overnight starter culture. Be sure to water your plants well the day before infiltration
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
at 3000 rpm for 25-30 minutes in a Beckman GS-R centrifuge. Each pellet then is washed with 30 ml of 70% ethanol and dried in vacuum. 10、Each pellet is dissolved in 210 ul of 10/0.1 TE by pipetting up and down and incubating at 37 degrees C followed
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