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- 2025年11月23日
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16315Glass vacuum filter holder, 25 mm/30 ml,
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day
Green lab protocol for vacuum infiltration transformation of Arabidopsis
Autoclave. Add antibiotics (30 g/ml works well for kanamycin). Pour into 150 X 15 mm plates. We also add vancomycin at 500mg/l
Green lab protocol for vacuum infiltration transformation of Arabidopsis
, inoculate a 500 ml culture of YEP medium containing 50 mg/l rifampicin, 25 mg/l gentamycin and the appropriate antibiotic for your construct with a 1 ml overnight starter culture. Be sure to water your plants well the day before infiltration
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