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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
11107-142------NCA Membrane_ 0.2µm_ 142mm_ 100pk
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文献和实验Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
(not Tris base). The pH is about 5. Don't adjust the pH. For yolk sac/placenta/tail/toes 2. Add 40 µl (100 µl for tail/toe) 25 mM NaOH / 0.2 mM EDTA. 3. Place at 95°C for 20 minutes (in a thermal cycler) 4. Add 40 µl (100 µl for tail/toe) 40 mM Tris
Preparation of Brain Membrane Fractions
in approximately 5 ml water. Add sucrose and water to give a final volume of 45 ml 1.2 M sucrose, add HEPES to 5 mM and the protease inhibitors as above. Homogenize in glass FEP polymer-homogenizer (3 strokes, 1000 rpm).Spin at 9200 x g for 20 min.Resuspend pellets
5 mM b-mercaptoethanol 390 µl TNE buffer for 1 L 25 mM Tris 3.03 g 100 mM NaCl 5.84 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b-mercaptoethanol 390 µl TEM buffer for 1 L 25 mM Tris 3.03 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b
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