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大量
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两年
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- 供应商:
科邦兴业(北京)科技有限公司
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盒
12303-142------GCA Membrane_ 1.2µm_ 142mm_ 25pk
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文献和实验Reagents : Bacterial strain E. coli N4830/pJW10 LB amp media 50 µg/ml ampicillin High salt buffer for 1 L 50 mM KH2PO4 6.8 g 150 mM KCl 11.18 g 50 mM sodium pyrophosphate (Na4P2O7-10H2O) 22.3 g 5 mM Na2 EDTA 1.86 g
Preparation of Brain Membrane Fractions
in approximately 5 ml water. Add sucrose and water to give a final volume of 45 ml 1.2 M sucrose, add HEPES to 5 mM and the protease inhibitors as above. Homogenize in glass FEP polymer-homogenizer (3 strokes, 1000 rpm).Spin at 9200 x g for 20 min.Resuspend pellets
DGK Membrane Preparation Contributor: Suprya Jayadev Date: Dec. 10, 1992 Reagents : Bacterial strain E. coli N4830/pJW10 LB amp media 50 µg/ml ampicillin High salt buffer for 1 L 50 mM KH2PO4 6.8 g
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