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9999
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广州威佳科技有限公司
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20片/盒
孔径:0.2μm
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文献和实验Steady State ATPase Assays Coupled Enzyme System
. 5. Make Tris-MgCl2 -DTT mix for all assays. Tris-MgCl2 -DTT mix = (n = # of assays + 1) n x 20 µL 0.5 M Tris-OAc, pH 7.5 20 µL 10 mM MgCl2 20 µL 10 mM DTT 70 µL DDW
FBS IMDM + 20% heat-inactivated FBS Viral transduction: Iscove's + 20% heat inactivated FBS, plus 100 ng/mL each of SCF, IL-6, and Flt-3L, plus 10 ng/mL IL-11. CFU assays: aMEM +30% FBS, 1% deionized BSA, 1.2% methylcellulose
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, 6.1g anhydrous Na2HPO4, 2g KH2PO4, H2O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.-
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