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Preparation of Brain Membrane Fractions

Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedures should be done at 4ºC using precooled reagents. For rat samples, immediately remove brain from the cranium into ice cold dissection buffer (50 mM Tris-acetate (TA

Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology

) 90°C hot plate Mask aligner (NXQ 4006, Neutronix‐Quintel) Photomask (design of the mask, usually 1‐ to 3‐µm features, can be generated by L‐edit Pro; Tanner EDA; quartz masks with the designed feature

Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions

7 ) 1 M lithium acetate (LiOAC; see recipe ) 10× TE buffer, pH 7.5: 100 mM Tris·Cl, pH 7.5, 10 mM EDTA (see appendix 2E ); store up to 1 year at room temperature