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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
50μg/1mg/500μg/10μg
| 规格： | 50μg | 产品价格： | ￥1680.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥7480.0 |
| 规格： | 500μg | 产品价格： | ￥4980.0 |
| 规格： | 10μg | 产品价格： | ￥780.0 |
产品信息
| 别名 | Fibroblast Growth Factor 1; FGF-1; Acidic Fibroblast Growth Factor; aFGF; Endothelial Cell Growth Factor; ECGFHeparin-Binding Growth Factor 1; HBGF-1; FGF1; FGFA |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Phe16-Asp155 |
| 检索号 | P05230 |
| 分子量 | 16 kDa |
| 表观分子量 | 16 kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.2-2 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 50mM MOPS, 100mM Na2SO4, 1mM EDTA, pH 7.9. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
-19 Human KGF 和100-52 Human FGF-23等。 2) 柠檬酸(盐)缓冲液 5mM柠檬酸钠(pH 8.0)用于溶解100-17A Human FGF-acidic, 10mM柠檬酸(pH 3.0)用于溶解100-21 Human TGF-b1等。 3) 醋酸溶液 10mM醋酸用于溶解120-10 Human Noggin和120-20 Human WISP-3等。 4) Tris缓冲液 5mM Tris(pH 7.6)用于溶解
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