sartorius赛多利斯11406--25------NC

In Situ Hybridization Using Digoxigenin Labeled Probes

as black bands. Weaker signals are brown Solutions: 1) 10X PBS 1.3 M NaCl 0.07 M Na2HPO4 0.03 M NaH2PO4 2) Hybridization Buffer 0.6 M NaCl 50 mM Sodium phosphate buffer, pH 6.8 1X Denhardt's (0.02% BSA / 0.02% Ficoll / 0.02

Construction and Characterization of Adenovirus Vectors

), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X

Methods for the preparation of Fly Extract (FE1 and FE2).

you may have to filter through 0.8µm and 0.45µm filters first. Aliquot to 2.5ml and freeze at -20°C.   Fly Extract 1 for initiating primary culture (FE1) 1. Procedure as for FE2 but the flies