Sartorius赛多利斯11107--50------NC

Preparation of Brain Membrane Fractions

.Use 20–50 µg protein per lane of mini SDS-polyacrylamide gels or 50–100 µg per lane of standard gel.Use any standard western blot protocol for immunodetection of glutamate receptor protein.B:Short Protocol: This is a time-saving protocol

DGK Membrane Preparation

Reagents : Bacterial strain E. coli N4830/pJW10 LB amp media 50 µg/ml ampicillin High salt buffer for 1 L 50 mM KH2PO4 6.8 g 150 mM KCl 11.18 g 50 mM sodium pyrophosphate (Na4P2O7-10H2O) 22.3 g 5 mM Na2 EDTA 1.86 g

[分享] Strip Antibodies and Re-use Protein blots membrane

20, or overnight with 3% BSA/Tween 20. Five： Stripping buffer: 0.5 L (sterile filter solution and keep at 4°C) 0.2 M Glycine, pH 2.5 0.05% Tween 20 Six： 50mM tris pH6.8, 100mM b-mercapto and 2% SDS. Leave on for 30 mins at 50C and then wash