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大量
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一年
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科邦兴业(北京)科技有限公司
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盒
sartorius赛多利斯11406--47------NCN white - black, non-sterile, 0.45 µm
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
as black bands. Weaker signals are brown Solutions: 1) 10X PBS 1.3 M NaCl 0.07 M Na2HPO4 0.03 M NaH2PO4 2) Hybridization Buffer 0.6 M NaCl 50 mM Sodium phosphate buffer, pH 6.8 1X Denhardt's (0.02% BSA / 0.02% Ficoll / 0.02
Construction and Characterization of Adenovirus Vectors
stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide
Methods for the preparation of Fly Extract (FE1 and FE2).
you may have to filter through 0.8µm and 0.45µm filters first. Aliquot to 2.5ml and freeze at -20°C. Fly Extract 1 for initiating primary culture (FE1) 1. Procedure as for FE2 but the flies
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