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大量
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两年
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- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
11104-142------NCA Membrane_ 0.8µm_ 142mm_ 100pk
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文献和实验Preparation of Brain Membrane Fractions
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedures should be done at 4ºC using precooled reagents. For rat samples, immediately remove brain from the cranium into ice cold dissection buffer (50 mM Tris-acetate (TA
5 mM b-mercaptoethanol 390 µl TNE buffer for 1 L 25 mM Tris 3.03 g 100 mM NaCl 5.84 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b-mercaptoethanol 390 µl TEM buffer for 1 L 25 mM Tris 3.03 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
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