赛默飞世尔Thermo Fisher Kapillarsäu

In-gel Tryptic Digest for Protein ID by Mass Spectrometry

or silver stained gel.Cut gel band into 1 mm cubes using clean razor blade on a clean glass surface.Transfer to an Eppendorf tube.2.Remove excess water with pipet.Add 25-35 μl acetonitrile to tube to cover gel pieces.Incubate 10 minutes at RT to dehydrate

Single Primer ("Semi-Random") P

in the second cycling regime. If that doesn't work, try changing primers. If problems persist, switch to the long method. Do the following PCR reaction: 15 µL H2O 3 µL 10xM-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted

25M3机械搅拌通风发酵罐的设计依据

为500mm，两端弯管总长度 9 iii冷却管总长度L计算：有前知冷却管总面积F=25.7㎡ 现取无缝钢管Ф51×2.0，每米长冷却面积为 则L= =172m 冷却管占有体积V=0.785×0.051 ×172=0.35m³ iv 每组管长 和管组高度： 另需连接管4m： =L+4=176m 可排竖直蛇管的高度，设为静液面高度，下部可伸入封头。 设发酵罐内附件占有体积为0.5m³，则： 竖直蛇管总高