赛默飞世尔Thermo Fisher Kapillarsäu

In-gel Tryptic Digest for Protein ID by Mass Spectrometry

or silver stained gel.Cut gel band into 1 mm cubes using clean razor blade on a clean glass surface.Transfer to an Eppendorf tube.2.Remove excess water with pipet.Add 25-35 μl acetonitrile to tube to cover gel pieces.Incubate 10 minutes at RT to dehydrate

Single Primer ("Semi-Random") P

in the second cycling regime. If that doesn't work, try changing primers. If problems persist, switch to the long method. Do the following PCR reaction: 15 µL H2O 3 µL 10xM-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge