赛默飞世尔Thermo Fisher HPLC column

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

for 6 hr at 55°C. (C ) Chromatogram of a commercial sample of uridine. Conditions: RP‐HPLC analysis is performed using UV detection (254 nm) and a 5 µm Supelcosil LC‐18S column (25 cm × 4.6 mm) according to the following method: starting from 0.1 M

Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands

 Reversed‐phase column: 125‐mm × 4‐mm, 5‐µm Lichrospher 100 RP18 (Merck), CC Nucleosil 100‐5 C18 (125/4; Macherey‐Nagel), or polystyrene  reversed‐phase (PRP‐1; Hamilton)

Uridine 2′‐Carbamates: Facile Tools for Oligonucleotide 2′‐Functionalization

, analytical grade Silica gel: 0.040‐ to 0.063‐mm Macherey‐Nagel Kieselgel 60 Chloroform (CHCl 3 ), HPLC grade, ethanol free