赛默飞世尔Thermo Fisher Fisherbrand
未灭菌塑料试管 TUBE W/O CAP 12X75MM 1000/PK 货号：T_70114-959-16- ￥70 - 1400
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货号：T_70114-959-16
中文名称：Fisherbrand 未灭菌塑料试管
英文名称：TUBE W/O CAP 12X75MM 1000/PK
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文献和实验Procedures for In Vitro Production of Bovine Embryos
in front of the heater) - Place 1-3 straws of semen in citothaw. - Layer semen on top of Percoll gradient. - Place Percoll tube in a warmed centrifuge canister. - Centrifuge for 15 min at 1000 x g. - Collect semen pellet with a Pasteur pipet
Southern Hybridization Protocols
). Mix gently by pipetting up and down and cap the tube. Spin for a few seconds in a microcentrifuge to bring the contents to the bottom of the tube.Incubate at 37℃ for 10 minutesStop the reaction by the addition of 2 m l 0.5% EDTA. For use
in 4% paraformaldehyde to give a working concentration of 10 µg/mL. Discard whatever is not used on the day of use. 6) Grid plates � Purchase from Fisher Scientific (#08-757-149) 7) Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain
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