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货号:T_7012780-16
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英文名称:EHRLICH RGT,UROBILINOGEN,500ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
antibody, in a moist chamber, at RT overnight (minimum for 2 hr.). Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 16- Wash twice in TBS-T. 17- add the AlkPhos-conjugated secondary antibody (100 to 200
, without CaCl2 , MgCl2 , MgSO4 , 500ml bottle, Store at 4° C. Glacial Acetic Acid: FISHER SCIENTIFIC cat# A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500ml bottle. L-Glutamine: GIBCO cat# 25030-032. L-Glutamine solution 100X (200mM), 20ml bottle
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