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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312789865
中文名称:
英文名称:X1000 Culture loop disposable sterile In bags of 50 10µL genpurpose polystyrene (pack of 1000)
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文献和实验Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
(Nunc 177402) Transfer pipettes, sterile disposable (Fisher) Tubes, sterile conical (15- and 50-mL) Tubes, sterile microcentrifuge (1.5-mL) Water bath preset to 37°C METHOD Dissection 1. Carefully remove
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube
9. Cover the openings of the deep well plates with Qiagen Airpore Tape Sheets and place the plastic lid over the sheet. Place the plates in a 37°C shaker incubator at 200 RPM for twenty-four hours. 10. Add 50 µl of 45% (w/v) sterile glycerol
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