60109-500-6-9赛默飞SLE 500MG/6ML

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

Wholemount staining of embryos

5'' washes with TBS. Use haematology mixer (these are really good mixers Fisher cat#14-060-1) Block protein binding sites by incubating embryos in TBS plus 2 mg/ml BSA plus o.1 (v/v) triton-X-100 plus 20 heat inactivated normal serum (donkey or goat

96-well RNA In Situ Hybridization Protocol

X Denhardts 7.5ml 10mg/ml tRNA 7.5ml 10mg/ml ssDNA (BOIL before adding) 0.3ml 50 mg/ml Heparin 3.0ml 10% Tween ****** ddH2 O 270ml Total volume ******Add 30ml H2 O to make hybridization buffer WITHOUT dextran sulfate. ******Add 30ml of 50