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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
that is very common in detritus in ponds and in the solid portion of sewage. The heavily granulated cytoplasm makes a very nice model for observing cytoplasmic streaming. Find the specimen using dark field mode at 40x. Chaos are very large, so this shouldn't
SYBRGreen Q-PCR in the ABI 7700
Materials ROX Passive Reference Dye 25 µM, 50x (Invitrogen, Cat. No. 12223-012 ) SYBR Green I, "10,000x" concentrate (Molecular Probes, Cat. No. S7563 ) SureStart Taq polymerase 5 U/µL (Stratagene, Cat. No. 600280 ) Solutions SYBR
Georgescu, R.E., Kim, S.S., Yurieva, O., Kuriyan, J., Kong, X.P. and O'Donnell, M. 2008. Structure of a sliding clamp on DNA. Cell 132:43‐54. Hollis, T. 2007
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