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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311797947
中文名称：
英文名称：DYE CONCENTRATE BLUE
货期：热销品现货，其他请咨询
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文献和实验. This treatmentwill remove some of the immobilized Cibacron Blue dye fromthe resin, so contact time should be minimized (see Note 4 ). 3.3.2. Step 2: IMAC Purpose: This step serves to remove contaminating proteins andto concentrate the volume of Wnt3A-containing
Protein Assay (Spectrophotometer)
volume up to 800µl with H2O. Add 200µl dye (Biorad Protein Assay Dye Reagent Concentrate) Cover cuvettes with parafilm and mix gently. The colour of sample (intensity of blue) should be between the two extremes of the standard curve. Avoid
soluble in the color reagent).The Bradford reagent should be a light brown in color.Filtration may have to be repeated to rid the reagent of blue components.The Bio-Rad concentrate is expensive,but the lots of dye used have apparently been screened
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