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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311702448
中文名称:
英文名称:X1000 SCREW CAP 13MM RUBBER/TEF
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
Array CGH of Microdissected Fresh or Formalin Fixed DNA
) 0.4 ml 0.5 M EDTA (PCR room) 183.6 m l dH2O 200 ml total 4) Sephadex column purification of labeled probe: Purify in Amersham G50 Column mix column by vortexing, snap off bottom, loosen cap, place column in 1.5 ml screw
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