赛默飞世尔Thermo Fisher X1020 GEL L

Clonality - X Chromosome Inactivation Assay

for 5 min at 94°C. Load 3 µl onto gel. Electrophorese at 1800 volts for 1 to 2 hours. TIP: In clonality analysis, the PCR products are ~200 bp. Therefore do not overload the gel with sample and run the gel for a longer period of time to sharpen

Radiolabeled sequencing gel preparation, loading, and electrophoresis

buffer, and immediately prior to loading each sample, flush the well with running buffer using gel loading tips. 9. Load 1-2 ul of sample into each well using a Pipetteman with gel-loading tips, and then electrophorese according the following

DNA fingerprinting - agarose gel

transparent trays. Insert comb. Use 200 ml of molten agarose. After gel is solidified, remove comb and place in electrophoresis unit with enough 1X TAE buffer to cover the gel. Load 1.75 µl of the restriction enzyme digestion/ loading dye mixture into gel