赛默飞世尔Thermo Fisher X400 Gel-Lo
ading Tips flat 200 / rack 0.4mm 1-200µ L (case of 400) 货号：T_70310421013- ￥1200 - 24000
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- 2025年07月13日
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货号：T_70310421013
中文名称：
英文名称：X400 Gel-Loading Tips flat 200 / rack 0.4mm 1-200µ L (case of 400)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
Gel-purified, agarased YACs for yeast transformation
in and out is fine.) ** Again, I skip step #18. 18. Determine concentration and analyze on pulsed-field gel. 19. Use all of one tube (50-100 µl) for LiAC transformation , always handling the DNA very gently and using cut-off pipette tips. Plate
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