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- 2025年07月15日
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文献和实验Monoclonal Antibody Production
) (3) 250 ml bottles of 100X penicillin-streptomycin (4) 10 ml of 100X HAT selection solution (5) 10 ml of 100X HT solution (6) 10 sterile flat-bottomed 96-well plates 3. Assaying for Positive Clones Run an ELISA.html">indirect ELISA using
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇：Antibody Conjugation Protocol 下一篇：Expanding Hybridoma Clones
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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