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- Thermo Fisher
- ER0271
- 赛默飞
- 2025年07月15日
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文献和实验Obtaining Embryos: hCG Injection
for comments about fertilization rates). The hCG (Chorulon) comes as a powder of 10,000 units that we resuspend in 10 ml of sterile water (final concentration 1 unit/microliter). For the priming dose, we dilute the stock of hCG ten fold and inject 100 µl
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Treatment 1. Add 20 μl of 10X CIP Buffer. 2. Add diluted CIP stock to the DNA such that the final concentration is approximately 0.2 Units CIP per μg DNA in a final volume of 200 μl (see Hint #4). 3. Incubate at 37°C
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