Sartorius赛多利斯14650--25------DP

【交流】关于NEB的T4 DNA连接酶和快速连接试剂盒的常见问题及解答

(4)。 一个韦氏单位=67个粘端连接活性单位。 浓度：400,000 units/ml和2,000,000 units/ml。 贮存条件： 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 µg/ml BSA和50%的甘油，-20°C贮存。 热失活条件: 65°C 加热10分钟。 室温连接反应：为方便起见，连接反应可以在室温（20-25°C）条件下进行。粘性末端连接：在20µl反应体系中加入1µl T4 DNA

体外荧光法检测核内体早期动力学

(laminar flow hoods, incubators, cooling centrifuges, water baths, refrigerator and freezers, cell culture plates and flasks) Plastic flasks (15-ml and 50-ml tubes and flasks of different sizes) and plastic pipettes (5, 10, 25 and 50 ml)

Preparation of Carboxypeptidase Y and Its Properties

℃ for 1 hr. In the presence of 10% methanol, the enzyme is completely stable at pH 5.5～8.0 for 8 hr at 25℃. Aqueous solu­tion of the enzyme is stable for 8 hr in the pH range 5.5～8.0 at 25℃ and for 2 hr in the pH range 6～8 at 37℃. At higher temperatures