赛默飞世尔Thermo Fisher ELECTROPHOR

Blue Native Gel Electrophoresis

Electrophoresis We use a Mighty Small Hoefer setup with a cooling core. Set a BN gel in a unit connected to a cooling circulator (2-4 C°C in the cathode tank) Load samples Run at constant voltage of 100-200 V, 3-6 hrs. For immunoblotting or fluorography:

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v