赛默飞世尔Thermo Fisher ELECTROPHOR
ESIS TANK HOEFER SE640 WIDE MINI-VERTIC al, dual, complete gel size 180mm(w) x 80mm(l) 货号：T_70311915358- ￥16000 - 320000
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货号：T_70311915358
中文名称：
英文名称：ELECTROPHORESIS TANK HOEFER SE640 WIDE MINI-VERTIC al, dual, complete gel size 180mm(w) x 80mm(l)
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文献和实验and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers
Blue Native Gel Electrophoresis
Electrophoresis We use a Mighty Small Hoefer setup with a cooling core. Set a BN gel in a unit connected to a cooling circulator (2-4 C°C in the cathode tank) Load samples Run at constant voltage of 100-200 V, 3-6 hrs. For immunoblotting or fluorography:
. Dissolve the precipitate in 100 x X ul dH2O (or 1/10 x TE) containing 1/10 x X ul RNase-It (Stratagene). Estimate the DNA concentration (by agarose gel electrophoresis with Etidium bromide or by Hoefer Dyna Quant 200) and make a solution adjusted at 100
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