赛默飞世尔Thermo Fisher 60 LITER PE

SSR GEL and Silver Staining Protocol

to first direction, don't use too much pressure. This treatment needs to be repeated every time.   III. GEL SOLUTION PREPARATION: 1. Rinse all glassware and plastic ware with d.i. water prior to gel solution preparation and pouring, including

SSR GEL and Silver Staining Protocol

for 2 min., ice and 5 μl 1X PE II buffer 2.5 μl of 400 ng / μl of sonicated Poly-dA 7.5 μl SSRP bufferVI. ELECTROPHORESIS:Note: Adding sodium acetate to the bottom reservoir during electrophoresis (Sheen and Seed, 1988, Biotechniques 6:942-944) produces

Patient‐Derived Models of Human Breast Cancer: Protocols for In Vitro and In Vivo Applications in Tumor Biology and Translational Medicine

, which enable use of bona fide uncultured human tissue in designing and validating cancer therapies. Curr. Protoc. Pharmacol. 60:14.23.1?14.23.43. © 2013 by John Wiley & Sons, Inc. Keywords: breast cancer; tumorgraft; organoid; 3D culture