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- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存;低温运输
- 英文名:
Deoxyribonuclease I
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
D128591-10KU/D128591-2.5KU
| 规格: | D128591-10KU | 产品价格: | ¥2522.90 |
|---|---|---|---|
| 规格: | D128591-2.5KU | 产品价格: | ¥694.90 |
基本描述
产品名称:DNA酶I
规格或纯度:EnzymoPure™, 来源于牛胰腺;≥2,000 units/mg dry weight;无Ribonuclease & Protease
产品介绍:
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Accession #:
级别:EnzymoPure™
产品规格参数
浓度:来源于牛胰腺;≥2,000 units/mg dry weight;无Ribonuclease & Protease
储存条件:2-8°C储存
运输条件:低温运输
CAS编号和信息:9003-98-9
功能和特点
Accession #:
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文献和实验High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
How to Make Simple Solutions and Dilutions
concentration = 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V 1 = (V 2 x C 2 ) / C 1 V 1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and cancel the units
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