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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human B7-H3 Protein(Site-Specific AF 647 Conjugation, ECD,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
25.00 µg/100.00 µg
| 规格: | 25.00 µg | 产品价格: | ¥3480.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥11180.0 |
蛋白名称:Human B7-H3 Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Thr 461) of human B7-H3 (Q5ZPR3-1) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE.
蛋白活性:1.Flow cytometric analysis of anti-B7-H3 CAR expression. 293 cells were lentivirally transduced with anti-B7-H3 CAR. Flow cytometric analysis was performed with a negative control protein and AF 647-conjugated human B7-H3 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11188-H86H-SG), respectively. Non-transduced 293 cells were used as a control (left). 2.Binding activity of AF 647-conjugated B7-H3 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 647-conjugated human B7-H3 protein (Site-Specific Conjugation, ECD, His Tag, Cat. No. 11188-H86H-SG) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Leu 29
蛋白分子量:The protein has a predicted molecular mass of 50 kDa.
蛋白NP号:Q5ZPR3-1
蛋白氨基酸序列:Met1-Thr461
蛋白标签:C-His
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