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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
MSI Analysis System, Version 1.1
- 供应商：
普洛麦格
- 规格：
100 reactions (50 reaction pairs)
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文献和实验The Polymerase Chain Reaction (PCR)
rather than Taq.How Big A Target? • Amplification products are typically in the size range 100-1500 bp. • Longer targets are amplifiable — >25 kb. • Requires modified reaction buffer, cocktails of polymerases, and longer extension times. • Limited
should be monitored initially at 30- to 60-sec intervals. To monitor the time course of the reaction: the beads three times each with 50 µL of 100 mM acetic acid. Add 30 µL of 100 mM acetic acid. Spot 0.5 µL of beads with 0.5 µl of MALDI matrix onto the MALDI
DNA Methylation Analysis by Bisulfite Sequencing (BS)
supernatant carefully, watching the tissue pellet. (note 1) Resuspend pellet by vortexing. Add 950 µL digestion buffer (100 µL 10X PCR buffer [100 mM TRIS, 15 mM MgCl2 , 500 mM KCl), 5 µL 0.5% Tween 20, 845 µL H2O) and 30-50 µL of 20 mg/ml Proteinase K
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