相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
T4 DNA Ligase
- 供应商:
普洛麦格
- 规格:
100u
| 说明 |
本产品为大肠杆菌中表达的克隆重组体。主要催化DNA上相邻的5`-磷酸基与3`羟基之间形成磷酸二酯键。 主要用途是连接带匹配粘端的DNA分子,或使平端的双链DNA分子互相连接或与合成接头相连接。也可以催化DNA与RNA之间以及少数RNA之间的连接,但不能催化单链DNA之间的连接。
|
|
| 活性单位定义 | 0.01u的T4 DNA连接酶可以在16℃下反应20分钟,连接95%的1µg λDNA-HindIII的分解物。
|
|
| 储存缓冲液 | 10mM Tris-HCl (pH7.0, 25℃),50mM KCl,1mM DTT,0.1mM EDTA和50%甘油。
|
|
| 10×反应缓冲液 | 300mM Tris-HCl (pH7.8, 25℃),100mM MgCl2 ,100mM DTT,10mM ATP。 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1 ). In addition to expanding the repertoire of functional groups
Splint Ligation of RNA with T4 DNA Ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase
技术资料暂无技术资料 索取技术资料









