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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
CC5 Internal Lane Standard 500 Y23
- 供应商:
普洛麦格
- 规格:
300μl
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文献和实验thin-walled PCR tubes on ice. For a 50 μL reaction, add: 2 μL Template DNA (10 ng-500 ng) 5 μl 10X Taq buffer with MgCl
Stewart Laboratory Standard Operating Procedure
9) Or anti-BrdU: Boeringher #1170376 1:10 dilution Wash cells once with 1.5 mL wash buffer. Resuspend cell pellet in anti-mouse FITC antibody (50 μL total solution with wash buffer, 1:500 dilution). Incubate for 20 minutes at 4°C. Wash cells
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
phenol/chloroform/isoamyl alcohol (25:24:1), vortex, centrifuge for 5 min and remove the supernatant to new tubes 11. Repeat step 10 12. Repeat this step with chloroform alone 13. Add 300 l isopropanol and incubate on ice for 10 min 14
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