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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
X-Gal
- 供应商:
普洛麦格
- 规格:
100mg/2 ml
基因。
特点:
• 浓度: 50mg/ml 的二甲基甲酰氨溶液, 2.0ml/ 瓶。
• 质量检测: X-Gal 通过使用 pGEM® -Z 载体的显色实验检验。
储存条件: 储存于 4℃或 -20℃。
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文献和实验, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
Materials Fixative 2% Paraformaldehyde (in PBS) Staining solution 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, 2 mM MgCI, 0.01% sodium deoxycholate 0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock 40 mg
Detection of -Galactosidase Activity: X-gal Staining
X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked
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