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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
CC5 Internal Lane Standard 500 Pro
- 供应商:
普洛麦格
- 规格:
300 μl
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
phenol/chloroform/isoamyl alcohol (25:24:1), vortex, centrifuge for 5 min and remove the supernatant to new tubes 11. Repeat step 10 12. Repeat this step with chloroform alone 13. Add 300 l isopropanol and incubate on ice for 10 min 14
玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用
5CS protein stained with Coomassie blue. Left lane, GST- P5CS; right lane, molecular weight markers.(C) and (D) Standard curve and its controlof Pi determination based on a malachite green colorimetric assay(E) Kinetic analysis ofP5CS was performed using
Mag-Bind® mRNA Protocol (Standard Protocol)
17. Aspirate the supernatant by pipetting. Remove the tube from magnetic stand. 18. Wash the magnetic beads by adding 300 μl mRNA Wash Buffer. Resuspend the magnetic beads by pipetting carefully a couple of times. 19. Collect
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