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- 2025年07月12日
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- 克隆性：
MOPC-21
- 标记物：
PE-Cy7
- 规格：
100 μg
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
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