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- BioGems
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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
1D3
- 标记物：
PE-Cy7
- 规格：
100 μg
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文献和实验thresholds for antigen receptors and other surface receptors on B cells; also regulates B cell development, activation and differentiation CD19 antibody inhibits B cell activation from anti-immunoglobulin Coreceptor with CD21 Earliest B cell
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Western Blotting with Horseradish Pe
for colorimetric detection, visualize the transferred proteins by staining the membrane for 15 minutes with India ink (Higgins black India ink, Eberhard Faber) diluted 1:1000 in wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Rinse excess stain with wash
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