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- BioGems
- 07131-50-100
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
RA3-6B2
- 标记物：
FITC
- 规格：
100 μg
Anti-Human/Mouse CD45R (B220) FITC 同型对照:Rat IgG2a, kappa标记:FITCwww.bio-gems.com/anti-human-mouse-cd45r-b220-fitc.html
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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