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- BioGems
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- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
N418
- 标记物：
PE-Cy7
- 规格：
25 μg
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文献和实验Western Blotting with Horseradish Pe
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10–15 seconds
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
TCA CAA CTT TAT TGC TGG 3' Length: 21-mer 5' 708 Tm: 64.6 °C (salt 1000.0 mM; oligo 100.0 pM) deltaG (25 °C): -38.8 kcal/mol Degeneracy: 1 P.E.#: 6/410 1/E: 5.06 nmol/A260 33.0 μg/A260 Z23168:948L21 Lower Primer 5' AGC CAT TTT CAA AGC CTC
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