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- 2025年12月14日
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- 英文名：
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- 库存：
现货库存
- 供应商：
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- 肿瘤类型：
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- 细胞类型：
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- 品系：
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- 组织来源：
ATCC/DSMZ/ECACC
- 相关疾病：
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- 物种来源：
人或动物
- 免疫类型：
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- 细胞形态：
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- 是否是肿瘤细胞：
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- 器官来源：
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- 运输方式：
常温或干冰
- 年限：
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- 生长状态：
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- 规格：
T25
| 种属 | 大鼠 |
| 组织来源 | 正常股动脉组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ；生长添加剂5ml ；胎牛血清50ml ；双抗5ml |
| 简介 | 股动脉是下肢动脉的主干 ，由髂外动脉延续而来。在腹股沟韧带中点的深面入股三角。在股三角内 ，股动脉先位于股 静脉的外侧 ，逐渐从外侧跨到股静脉的前方 ，下行入收肌管 ，再穿收肌腱裂孔至腘窝 ，易名腘动脉。股动脉在腹股沟 中点处位置表浅 ，可摸到搏动 ，是临床上急救压迫止血和进行穿刺的部位。股动脉动脉内皮细胞组成了动脉内壁 ，并 持续受到血流剪切应力的影响。内皮细胞在切应力的作用下 ，分泌不同的内皮因子并进而影响血管收缩和生长。主动 脉内皮细胞也调节细胞黏附分子的表达来控制和精确调节炎症反应和组织纤维化。体外培养的原代股动脉内皮细胞可 有效地帮助研究者研究内皮功能失调的机理 ，动脉粥样化等疾病的发病机理以及发展新的治疗方法。 |
| 形态 | 长梭形细胞样 ，不规则细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 平滑肌肌动蛋白( α -SMA ）免疫荧光染色为阳性免疫荧光鉴定 ，细胞纯度可达90%以上 ，不含有HIV-1、HBV、 HCV、支原体、细菌、酵母和真菌等。 |
cell lysis since IL-2-stimulated NK cells lkdiated tumor cell destruction in vitro. The necessary function of NK cells was further validated in vivo through selected antibody depletion of NK cells, which resulted in an overwhelming lung tumor burden relative to that in animals receiving a control rabbit IgG depletion regilkn. Interestingly, mice achieved
therapies.The blockage of intersectional communication between tumor and its metabolic and immune microenvironment is now considered a promising solution in treating cancer. Tumors have been identified as a special type of 'wounds' that do not heal. Recent studies demonstrate that the lack of the transforming growth factor beta (TGFB) signaling pathway in CD4+ helper T
Bone metastasis are the most common cause of pain related to cancer, reducing patients quality of life, and sometimes threatening their life-expectancy. Their management has to be pluridisciplinary, because of all the therapeutic options and the diversity of bone metastasis locations. The aim of this work is to propose a rational decisional algorithm for the treatment strategy of these secondary locations. Anti-resorbtive drugs with systemic action, surgery, conventional or stereotactic radiation therapy, and new techniques of interventional radiology are options that could be used separately or combined. They have shown benefits on symptomatic treatment, improving quality of life. Their indications vary according to the tumor site (short vs. long bones, carrier vs. non-carrier bones), the symptomatology (pain, neurologic symptoms), and the presence of complications (most of all fractures). The diverse presentations lead us to define this decisional algorithm, to guide the practice, while giving the maximal benefit to each patient according to each metastasis.
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文献和实验cell lysis since IL-2-stimulated NK cells lkdiated tumor cell destruction in vitro. The necessary function of NK cells was further validated in vivo through selected antibody depletion of NK cells, which resulted in an overwhelming lung tumor burden relative to that in animals receiving a control rabbit IgG depletion regilkn. Interestingly, mice achieved
贴壁法： 器械 显微器械：眼科剪一把，直弯眼科镊各一把，细结镊一把，手术刀一把，针头诺干 杀老鼠器械：手术剪一把，大镊子一把，弯眼科镊一把，组织钳一把，50ml烧杯一个（锡纸包好） 另外：10ml离心管两个，培养皿两个，10ml的瓶子三个，小滤器两个，硅胶板一块，培养瓶盖子诺干 贰。试剂 D-HANKS液250ml，双抗2ml（青霉素16万U/ml链霉素15万U/ml）。FBS和含20％FBS的DMEM 叁。步骤 1． 取大鼠，称重，水合
我养了快半年的大鼠气道平滑肌细胞，有一点体会，特发上来和各位战友交流一下，互相进步。 壹． 器械 显微器械：眼科剪一把，直弯眼科镊各一把，细结镊一把，手术刀一把，针头诺干 杀老鼠器械：手术剪一把，大镊子一把，弯眼科镊一把，组织钳一把，50ml烧杯一个（锡纸包好） 另外：10ml离心管两个，培养皿两个，10ml的瓶子三个，小滤器两个，硅胶板一块，培养瓶盖子诺干 贰。试剂 D-HANKS液250ml，双抗2ml（青霉素16万U/ml链霉素15万U/ml），一型胶原
PriCells –正常大鼠原代主动脉平滑肌细胞培养一、实验试剂1、培养基： PriCells Medium + 10% FBS + 1% P/S + PriCells Supplement2、冻存液： PriCells Medium + 20% FBS + 10% DMSO3、洗涤液： 1 × PBS （pH 7.4 ）+ 1% P/S4、染色液： 0.4% Trypan Blue5、消化液： PriCells Isolation of Primary Cell Kit6、检测试剂：肌动蛋白
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