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- 文献和实验
- 技术资料
- 英文名:
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- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
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- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
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- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
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- 生长状态:
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- 规格:
T25
| 种属 | 小鼠 |
| 组织来源 | 正常肺动脉组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清25ml ;双抗5ml |
| 简介 | 肺动脉血管内皮细胞是一种多功能细胞 ,尤其在肺的非呼吸功能方面更具特殊意义。当其受损 ,肺血管通透性升高导 致肺水肿 ,在成年人呼吸窘迫综合症发生机制中具有重要意义。但是处于体内多因素共同作用的复杂环境中 ,对肺血 管内皮细胞的功能和代谢以及病变发生机制很难作深入研究。原代肺动脉内皮细胞的体外培养系统有助于在特定的体 外条件下研究肺血管内皮细胞的功能。 |
Xia H, Riaza M, Zhang MY, Liua B, El-Desoukia Z, Jiang C C. Biochar increases nitrogen use ef ficiency of maize by relieving aluminum toxicity and improving soil quality in acidic soil[J]. Ecotox Environ Safe,P-ATV can be easily manufactured within a few days and ewdtciently boost neoepitope-specific CD8+ T cells to activate personalized immunotherapy. This simple and powerful approach of engineered ATVs provides an alternative strategy for personalized immunotherapy and is readily
Results: Forward flushing of the filter removed 85% to 95% of residual RBCs and platelets. When backward flushed with 800 mL, 95% of the WBCs recovered were contained in the first 400 mL. The number of recovered WBCs was in the range of 166-211 million/100 mL filtered blood. Subpopulations of WBCs recovered from the LRFs were in the same proportion as the donors whole blood. Viability of recovered WBCs was 96-99%. Exogenous rabbit antibodies bound well to the recovered WBCs and were retained for at least 5 h without significant reduction. Three full scale runs of WBCs recovered from donor blood filtered through the LRF met all FDA specification of sterility, endotoxin levels, viability and stability.
Conclusion: Using LRFs, high quality clinical grade WBCs are readily obtained in quantities of 0.2 to 1.2 billion cells from 100 mL to 450 mL (1 unit) of whole blood.
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文献和实验/Lone AH, Najar G R, Ganie M A, Sofi JA,Tahir Ali T. Biochar for Sustainable Soil Health: AReview of Prospects and Concerns[J]. Pedosphere, 2015, 25(5): 639~653.
培养基。放入37℃,5%CO2培养箱中培养。72h后细胞从环内外生长迁出,环内为内皮细胞,环外为成纤维细胞。将动脉环除去后,可看到内膜面细胞集落与外膜面之间有明显的无细胞区界限,用玻璃针剔除成纤维细胞,得到纯的内皮细胞生长克隆。继续培养10~15d,此时FBS浓度为20%,形成细胞单层。 4、置于5%CO2孵箱中培养,每隔3天换液,培养时添加15% FBS,5~10μg/ml的内皮细胞生长因子(ECGF)以及100μg/ml的肝素。待细胞90%~95%融合时,0.25胰蛋白
实验材料: 1. 正常大兔主动脉; 2. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素,pH7.2; 3. 培养用液:M199培养液(含20%小牛血清);0.125%胰蛋白酶-0.01%EDTA(1:1,V:V)混合消化液;D-Hanks液、100IU/ml青霉素和100μg/ml链霉素; 4. 培养器具:培养瓶或皿、白内障、眼科剪、镊子等; 培养方法: 1. 将动物主动脉取出
实验材料: 1. 大鼠主动脉血管; 2. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素,pH7.2; 3. 培养用液:M199培养液或RPMI1640培养液(含20%小牛血清,pH7.2);0.125%胰蛋白酶-0.01%EDTA(1:1,V:V)混合消化液;D-Hanks液、100IU/ml青霉素和100μg/ml链霉素;1%明胶溶液; 4. 培养器具:培养瓶或皿、白内障、眼科剪、镊子等; 培养方法: 1. 将大鼠
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