相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
/
- 肿瘤类型:
/
- 细胞类型:
/
- 品系:
/
- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
/
- 物种来源:
人或动物
- 免疫类型:
/
- 细胞形态:
/
- 是否是肿瘤细胞:
/
- 器官来源:
/
- 运输方式:
常温或干冰
- 年限:
/
- 生长状态:
/
- 规格:
T25
| 种属 | 兔 |
| 组织来源 | 正常眼组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清10ml ;双抗5ml |
| 简介 | 巩膜是眼球壁的最外一层 ,由致密的胶原和弹力纤维构成 ,其结构坚韧 ,不透明。巩膜前缘接角膜缘 ,后方与视神经 的硬膜鞘相延续 ,巩膜由外向内分为 :巩膜上层;主质层;巩膜下层。其中上层是由上皮细胞构成。 |
| 形态 | 上皮细胞样 ,不规则细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 广谱角蛋白(PCK)免疫荧光染色为阳性免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、HCV、支原 体、细菌、酵母和真菌等。 |
The median interval from symptom onset to diagnosis was eight weeks. The minimum follow-up duration was four months. Three patients had no history of malignancy. Of the remaining seven patients, three had other known metastases. Six patients underwent solely palliative radiotherapy. Three patients had amputation. One was treated with surgical excision and radiotherapy. One had an amputation and axillary node clearance. All but one patient had died by the time of the latest follow-up appointment. The median time to death following identification of acrometastases was 18 months. Sites of primary disease were skin (n=4), lung (n=3), kidney (n=2) and neuroendocrine system (n=1). The thumb was the most commonly affected location. Conclusions This study demonstrates that patients presenting with non-mechanical hand pain should be considered to have a malignant process until proved otherwise, particularly in patients with thumb symptoms and a history of prior malignancy.
Zhao M Y, Zhao Y, Xie L, Zhang G G, Wei Z M, Li J, Song C H. The effect of calcium superphosphate addition in different stages on the nitrogen fixation and ammonification during chicken manure
composting[J]. Bioresource Technol, 2023, 374, 128731.
scientists from academia, industry and regulatory agencies from around the globe to interact and exchange important scientific advances related to tumor immunobiology and cancer
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验/Zhao M Y, Zhao Y, Xie L, Zhang G G, Wei Z M, Li J, Song C H. The effect of calcium superphosphate addition in different stages on the nitrogen fixation and ammonification during chicken manure
composting[J]. Bioresource Technol, 2023, 374, 128731.
实验材料: 1. 2—3月龄的家兔胆囊; 2. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素,pH7.2; 3. 培养用液:以DMEM/F12为基础培养基,添加10%的胎牛血清、2 mmol/L谷氨酰胺、2μg/ml胰岛素、10 ng/ml表皮生长因子以及100 IU/ml青霉素和100μg/ml链霉素。酶消化液为0.125%Ⅳ型胶原酶溶液。组织清洗液为DMEM培养液,并添加200 IU
实验材料: 1. 大兔的正常食管组织 2. 6孔培养板:用多聚赖氨酸4℃包被过夜 3. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.4 4. 手术刀、解剖剪、解剖镊、眼科剪,眼科镊 实验方法: 1. 处死大兔,取正常食管组织放入含双抗的PBS(pH=7.2)中反复清洗3次,以洗去组织表面
片而不使囊膜片漂浮,每周换液2次。晶体上皮细胞在接种3天后,在倒置显微镜下可见,植块边缘有新生的细胞爬出,细胞呈规则六边形,大小均匀,胞质透明。一周后可融合成小片细胞。二周后可长满融合成单层细胞。细胞多为类圆形、多角形。2、传代培养:细胞铺满瓶底或生长到一定密度时,用0.25%胰蛋白酶消化,1∶2传代培养。传代时在培养瓶或培养皿底放置适当大小的盖玻片,细胞生长其上,便于光镜及免疫组化观察。细胞一般24h内贴壁,约3~4d细胞可达融合,融合的细胞和原代相似,大小基本一致。传代超过4~6代时,细胞的增长
技术资料暂无技术资料 索取技术资料








