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- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
/
- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
/
- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
/
- 生长状态:
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| 种属 | 人 |
| 组织来源 | 颈静脉球瘤组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清50ml ;双抗5ml |
| 简介 | 颈静脉球瘤是指起源于颈静脉球体外膜以及沿迷走神经耳支和舌咽神经鼓室支等部位分布的副神经节肿瘤。按肿瘤生 长的部位 ,通常将发生于颅底颈静脉孔及其附近者称为颈静脉球体瘤 ,发生于中耳鼓室者称为鼓室球瘤 ,但临床因经 常难以确定肿瘤的原发部位 ,故常将二者统称为颈静脉球瘤。 |
| 形态 | 梭形细胞样 ,不规则细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | S100免疫荧光染色为阳性免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、HCV、支原体、细菌、酵 母和真菌等。 |
| 倍增时间 | 每周 2 至 3 次 |
| 换液频率 | 2-3天换液一次 |
| 培养条件 | 气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液 :90%FBS ,DMSO 10%, 或使用非程序冻存液 :官网货号JY-H040 |
| 备注 | 人颈静脉球瘤细胞永生化通过慢病毒转染的方式携带SV40基因。 |
| 产品使用 | 仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
treated in the Second Aff iliated Hospital of Hebei University of Traditional Chinese Medicine were retrospectively selected,in addition,126 people ( control group) who received routine physical examination due to headache and other reasons were selected.And the general data of the two groups were collected and compared.The levels of serum HMGB -1 and sRAGE were measured by enzyme-linked immunosorbent assay ( ELIS
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文献和实验/treated in the Second Af filiated Hospital of Hebei University of Traditional Chinese Medicine were retrospectively selected,in addition,126 people ( control group) who received routine physical examination due to headache and other reasons were selected.And the general data of the two groups were collected and compared.The levels of serum HMGB -1 and sRAGE were measured by enzyme-linked immunosorbent assay ( ELIS
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