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- 2025年07月11日
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文献和实验Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible
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